Book title: Chiral separations: methods and protocols
Editors: Gübitz G, Schmid MG
Publisher: Humana Press Inc.
Series title: Methods in molecular biology
Volume number: 243
Abstract: It is strongly recommended for the reader to first study the introduction in Chapter 9, "Chiral Separations by HPLC Using Molecularly Imprinted Polymers," to extract the basic features off molecularly imprinted polymer (MIP) synthesis. Also, it can be useful to read the Methods section and to study the notes where practical information can be found that complements the information given in this chapter.
The capillary columns used in capillary electrochromatography (CEC) and capillary liquid chromatography offer several advantages over conventional columns, including lower sample consumption and, often, faster analysis times. However, packing a thin (20–100 mm) capillary with an ordinary particle-based material can be tricky and time-consuming. When preparing packed capillary columns, there is a need for retaining frits, and these might cause, e.g., band broadening and sample adsorption. Recently, much effort has been put into developing new types of stationary phase materials for the capillary format. One of the more successful approaches is the monolithic format. Using this technique, the stationary phase is prepared in situ, i.e., inside the capillary. The monolith can either be synthesized de novo or constructed by entrapment of prefabricated particles. When synthesizing the monolith de novo, particle packing procedures and the need for retaining frits are circumvented. The monolith has a sponge-like structure, which can be designed to provide a high porosity, stability, and flexibility. Also, stationary phases coated on the inner surface of the capillary for open tubular (OT) applications have been identified as promising stationary phase types for the capillary format. The synthesis and use of MIP monoliths and coatings for CEC will be covered in this chapter.
CEC has become known as a powerful tool in analytical chemistry, and it has been identified as a technique able to improve separations based on MIP sta-tionary phases. Synthesis of MIPs has been described previously. In short, MIPs are synthesized utilizing a template-assisted polymerization, where the monomers assembled around the template are polymerized, together with cross-linking monomers, to create a cavity in the polymer that is complementary to the template in shape, size, and chemical functionality. This cavity is able to recognize and rebind the template (analyte) with high selectivity.