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Reference type: Journal
Authors: Guo TY, Xia YQ, Wang J, Song MD, Zhang BH
Article Title: Chitosan beads as molecularly imprinted polymer matrix for selective separation of proteins.
Publication date: 2005
Journal: Biomaterials
Volume: 26
Issue: (28)
Page numbers: 5737-5745.
DOI: 10.1016/j.biomaterials.2005.02.017
Alternative URL: http://www.sciencedirect.com/science/article/B6TWB-4FX23J8-2/2/6bd9a3288734dc199c4f0e130d2231c5

Abstract: Two kinds of molecularly imprinted polymers were prepared using hemoglobin as the imprinting molecule, acrylamide as the functional monomer, chitosan beads and maleic anhydride-modified chitosan beads as matrixes, respectively. Static adsorbing experimental results showed that an equal class of adsorption was formed in the imprinted polymers and the adsorption equilibrium constant and the maximum adsorption capacity were evaluated. Chromatographic characteristics showed that the column bedded with the hemoglobin imprinted beads could separate hemoglobin and bovine serum albumin effectively from their mixture, which indicates that the imprinted beads have very higher selectivity for hemoglobin than the non-imprinted with the same chemical composition
Template and target information: protein, hemoglobin
Author keywords: Chitosan beads, polyacrylamide, Protein separation, molecular imprinting

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