MIPs logo MIPdatabase      Special issue call for papers   
Custom Search
Reference type: Journal
Authors: Silvestri D, Cristallini C, Ciardelli G, Giusti P, Barbani N
Article Title: Molecularly imprinted bioartificial membranes for the selective recognition of biological molecules. Part 2: Release of components and thermal analysis.
Publication date: 2005
Journal: Journal of Biomaterials Science-Polymer Edition
Volume: 16
Issue: (3)
Page numbers: 397-410.
DOI: 10.1163/1568562053654130

Abstract: Molecularly imprinted membranes imprinted for a large- molecular-weight protein were realised using a blend of natural and synthetic polymers. Bioartificial membranes of synthetic (poly(ethylene-co-vinyl alcohol)-EVAL, Clarene((R))) and biological (Dextran) polymers, molecularly imprinted with α-amylase as the template, were prepared and investigated. Dimethyl sulfoxide (DMSO) solutions of the alpha-amylase template, Clarene and Dextran were mixed under stirring in the desired proportions and dipped in DMSO (solvent)/water (non solvent) mixture, to obtain the phase separation. The release of Clarene, Dextran and α-amylase in the inversion baths was quantified by spectrophotometric methods and final composition of membranes was established. To study the interactions between the polymer components and between polymeric materials and the template, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) were carried out. Results indicated that stable and continuous bioartificial membranes of Clarene and Dextran can be obtained, whereby calorimetric analysis suggested the presence of high interaction between alpha-amylase and the Clarene component
Template and target information: protein, α-amylase
Author keywords: molecular imprinting, protein recognition, bioartificial materials, phase inversion

  Bag featuring the name Caterina spelled out in symbols of the chemical elements  Periodic table Bag bag  Meerkat somebody mention coffee mug


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner