MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Kriegel T, Schellenburger W, Kopperschlager G, Hofmann E
Article Title: Yeast phosphofructokinase - kinetic characterization of a substrate-imprinted enzyme conformation.
Publication date: 1991
Journal: Biomedica Biochimica Acta
Volume: 50
Issue: (12)
Page numbers: 1159-1165.

Abstract: Intramolecular cross-linking of octameric yeast phosphofructokinase was applied to study the effect of substrate-imprinted conformational changes on the regulatory properties of the enzyme:Cross-linking performed in the presence of fructose 6-phosphate yields a substrate-imprinted enzyme species the affinity of which towards this substrate is significantly higher than that of native phosphofructokinase and of the enzyme cross-linked in the absence of fructose 6- phosphate. The enzyme cross-linked in the presence of fructose 6-phosphate does not exhibit cooperativity with respect to this substrate but is still activated by AMP and by fructose 2,6- bisphosphate. This activation consists in an increase of substrate affinity with respect to fructose 6-phosphate. In the absence of positive effectors, the maximum activity of the cross-linked enzyme corresponds to the respective values of native phosphofructokinase when activated by AMP or by fructose 2,6-bisphosphate. At saturating levels of AMP and of fructose 2,6-bisphosphate, nearly identical affinities with respect to fructose 6-phosphate are found, ranging between the K(m) values of native phosphofructokinase activated by AMP and by fructose 2,6-bisphosphate. Covalent stabilization of the substrate- imprinted enzyme conformation does not affect the interaction of phosphofructokinase with ATP at the substrate-binding site. The results suggest that the allosteric regulation of yeast phosphofructokinase is mainly related to conformational changes controlled by fructose 6-phosphate while the ATP affinity at the catalytic site of the enzyme remains essentially unaffected

  SMI cryptic logo shirt, new improved design  SMI logo mug  Woman of proper-tea mug

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner