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Reference type: Journal
Authors: Dong H, Tong AJ, Li LD
Article Title: Syntheses of steroid-based molecularly imprinted polymers and their molecular recognition study with spectrometric detection.
Publication date: 2003
Journal: Spectrochimica Acta Part A-Molecular and Biomolecular Spctroscopy
Volume: 59
Issue: (2)
Page numbers: 279-284.
DOI: 10.1016/S1386-1425(02)00179-8

Abstract: Recognition of five steroid compounds, b-estradiol, ethynylestradiol, estradiolbenzoate, testosterone and methyltestosterone were studied using a synthesized molecularly imprinted polymer (MIP). When b-estradiol was used as the template molecule, the polymer was synthesized with methacrylic acid (MAA) as the functional monomer and ethylene glycol dimethacrylate (EGDMA) as the cross linking agent through. non- covalent interactions. It is found that the kind of porogen solvent and the polymerization conditions greatly affected the binding ability of a MIP to a certain molecule. Releasing of the template was performed by continuous extraction with methanol containing 10% acetic acid in a Soxhlet extractor. Our results indicated that such carefully synthesized MIP showed specific affinity toward b-estradiol in the adsorption process. (C) 2002 Elsevier Science B.V. All rights reserved

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