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Reference type: Journal
Authors: Russell AJ, Klibanov AM
Article Title: Inhibitor-induced enzyme activation in organic-solvents.
Publication date: 1988
Journal: Journal of Biological Chemistry
Volume: 263
Issue: (24)
Page numbers: 11624-11626.
Alternative URL: http://www.jbc.org/cgi/reprint/263/24/11624

Abstract: The enzymatic activity of the protease subtilisin in anhydrous organic solvents can be dramatically increased by pretreating the enzyme before it is placed in the nonaqueous medium. For instance, lyophilization of subtilisin from aqueous solution containing competitive inhibitors (followed by their removal) created an enzyme which was up to 100 times more active than the enzyme lyophilized in the absence of such ligands. This phenomenon of ligand-induced "enzyme memory" also extends to the stability, affinity, and substrate specificity of subtilisin in organic solvents.

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