MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Shnek DR, Pack DW, Sasaki DY, Arnold FH
Article Title: Specific protein attachment to artificial membranes via coordination to lipid-bound copper(II).
Publication date: 1994
Journal: Langmuir
Volume: 10
Issue: (7)
Page numbers: 2382-2388.
DOI: 10.1021/la00019a058

Abstract: A versatile and convenient method for targeting proteins to lipid assemblies using metal ion coordination is described. Mixed lipid bilayers and Langmuir monolayers containing a metal-chelating lipid and divalent copper ions are shown to bind protein via surface-accessible histidine residues. Cu2+ chelated by iminodiacetate (IDA) in the headgroup serves as an affinity ligand to target the protein to the interface. The compact, uncharged Cu2+-IDA headgroup can be incorporated into lipid assemblies without disrupting the lipid packing. Surface pressure-area isotherms of DSPC monolayers containing 5 mol % of IDA-lipid show that Cu2+ enhances the rate and extent of myoglobin association with the interface. Myoglobin binds to small unilamellar vesicles containing 2% Cu2+-IDA lipid (48% DSPC and 50% cholesterol) at least an order of magnitude more tightly than to vesicles without metal or loaded with Ca2+. The Cu2+-IDA lipid more than doubles the amount of protein targeted to the interface. Cu2+ ESR parameters g(parallel-to) and A(parallel-to), measured for liposomes with native and DEPC- modified myoglobin, support coordination of surface histidine side chains to Cu2+ as the binding interaction


  Apron featuring the name Frances spelled out in symbols of the chemical elements  Lab Rats Periodic table mug  HE-MAN polymer chemistry shirt

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner