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Reference type: Journal
Authors: Tremblay M, Voyer N
Article Title: Enhanced activity of a-chymotrypsin in organic media using designed molecular staples.
Publication date: 2005
Journal: Tetrahedron
Volume: 61
Issue: (28)
Page numbers: 6824-6828.
DOI: 10.1016/j.tet.2005.04.063

Abstract: We report the enhancement of a-chymotrypsin activity in organic solvents using modified peptides bearing two crown ethers. The transesterification of N-acetyl-L-phenylalanine ethyl ester with 1-propanol was used as model reaction. Co-lyophilization of crown ether modified peptides with a-chymotrypsin prior to use resulted in an increase of enzyme catalytic activity in non-aqueous media. The efficiency of enzyme activation is dependent on the amino acid sequence of peptidic additives and on the positions of the amino acids bearing the crown ligand
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