Abstract: A potentiometric chemosensor for selective determination of dipicolinic acid (2,6-pyridinedicarboxylic acid, DPA) , a biomarker of endospores, was developed based on the surface imprinting technique coupled with a nanoscale transducer: an indium tin ox ide ( ITO )-coated glass plate. A polysiloxane film was covalently bound onto an ITO surface in the presence of DPA after the simultaneously self-assembling octadecyltrichlorosilane and DPA . The sensor fabrication conditions, optimal recognition condition, as well as selectivity , sensitivity and stability of the DPA sensor have been investigated. The DPA sensor could recognize DPA ( 2 ,6-pyridinedicarboxylic acid) from other pyridinedicarboxylic acids. Potentiometric measurements demonstrated selective detection of DPA in a concentration range of 1.5 x 10-6 - 0.0194 M . The response time of DPA sensor for 4 x 10-4 M DPA was 25s. The potentiometric response of the DPA sensor to DPA is at 90 % of its initial magnitude after 550 times measurement. The viability of such a modified ITO electrode in the presence of other inorganic, organic and biological materials was probed.