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Reference type: Conference Proceeding
Authors: Minoura N, Ogiso M
Publication date: 2002
Article title: Syntheses and properties of temperature-responsive polymer gels with protein recognition sites by using the molecular imprinting technique.
Page numbers: 1706
Alternative URL: http://www.jstage.jst.go.jp/article/spsjppj/51m/0/51m_1706/_article

Proceedings title: Polymer Preprints, Japan
Volume number: 51m

Abstract: We prepared a temperature-response polymer gel with recognition sites for glucose oxidase (GOD) by the molecular imprinting technique. The polymer gel was composed of N-isopropylacrylamide, acrylamide and crosslinking agents. To assess the GOD-selectivity of this polymer gel, a competitive adsorption of the template protein (GOD) versus a non-template protein [glucose-6-phosphatedehydrogenase (G6PD)] was assayed. The GOD selectivity fo the polymer gel was 1.8 times higher than that of G6PD. It suggests that the temperature-responsive polymer with protein recognition sites can separate the template protein from a protein mixture.


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