Book title: Proteomics and Peptiodomics: New Technology Platforms Elucidating Biology
Editors: Marko-Varga G
Series title: Comprehensive Analytical Chemistry
Series editors: Barceló D
Volume number: 46
Abstract: This chapter focuses on the field of electromigration separation techniques for peptide and protein analysis. Capillary electrophoresis (CE) is one of the most promising analysis techniques in the field of separation science, which offers the advantages of short analysis time and minimum consumption of both reagents and samples. Capillary electrochromatography (CEC) is the latest separation technique, a modern combination of chromatography and electrophoresis, which is well suited to capillary format and miniaturized separation systems. Electrochromatography uses an electroendosmotically driven flow to propel the mobile phase through the column instead of a pressure-driven flow generated by expensive and seldom pulse-free pumps. The electroendosmotic flow is generated in the electrical double layer at the capillary inner wall or the charged solid-liquid interfaces. The number of both technological and methodological approaches increases every day as the interest in proteomics is growing among the researchers. Although many difficulties related to CE and CEC are waiting for solution, electromigration separation techniques belong to the most potent tools, especially when they are hyphenated with mass spectrometry in proteomics today.