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Reference type: Journal
Authors: Zhang ZH, Long YM, Nie LH, Yao SZ
Article Title: Molecularly imprinted thin film self-assembled on piezoelectric quartz crystal surface by the sol-gel process for protein recognition.
Publication date: 2006
Journal: Biosensors and Bioelectronics
Volume: 21
Issue: (7)
Page numbers: 1244-1251.
DOI: 10.1016/j.bios.2005.05.009
Alternative URL: http://www.sciencedirect.com/science/article/B6TFC-4GG2HY6-1/2/c84f1ba178127764858a52b756527bc2

Abstract: A novel method of combining sol-gel and self-assembly technology to prepare a human serum albumin (HSA)-imprinted film on the surface of piezoelectric quartz crystal (PQC) Au-electrode modified with thioglycolic acid was described in this paper. The imprinting process was characterized by using the piezoelectric quartz crystal impedance (PQCI) technique and electrochemical impedance technique. Scanning electron microscope (SEM) was employed to characterize the surface morphology of the resultant imprinted film. The piezoelectric technique and electrochemical impedance technique were also employed to investigate the binding performance of the sol-gel-imprinted film with the template protein. The results showed that the imprinted PQC film can give selective recognition to the template protein. The effects of salts and solvents on the binding capacity of the imprinted film with protein were discussed in detail. Other influencing factors (temperature and pH) have also been investigated. This self-assembly sol-gel imprinting technique was proved to be an alternative method for the preparation of biomacromolecule-imprinted thin film
Template and target information: protein, human serum albumin, HSA
Author keywords: molecularly imprinted, sol-gel, Self-assembled, protein, piezoelectric quartz crystal


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