MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Fu Q, Sanbe H, Kagawa C, Kunimoto KK, Haginaka J
Article Title: Uniformly sized molecularly imprinted polymer for (S)-nilvadipine. Comparison of chiral recognition ability with HPLC chiral stationary phases based on a protein.
Publication date: 2003
Journal: Analytical Chemistry
Volume: 75
Issue: (2)
Page numbers: 191-198.
DOI: 10.1021/ac026039z

Abstract: Uniformly sized molecularly imprinted polymers (MIPs) for (S)- nilvadipine have been prepared by a multistep swelling and polymerization method using methacrylic acid, 2- (trifluoromethyl)acrylic acid, 2-vinylpyridine, or 4- vinylpyridine (4-VPY) as a functional monomer and ethylene glycol dimethacrylate (EDMA) as a cross-linker. The chiral recognition abilities of the MIPs for nilvadipine and other dihydropyridine calcium antagonists were evaluated using a mixture of sodium phosphate buffer (or water) and acetonitrile or only acetonitrile as the mobile phase. The (S)-nilvadipine- imprinted 4-VPY-co-EDMA polymers gave the highest resolution for nilvadipine among the MIPs prepared. In addition, the enantio-separation of nilvadipine was attained using the (S)nilvadipine-imprinted EDMA polymers, without use of a functional monomer. H-1 NMR and molecular modeling studies suggested a one-to-one hydrogen-bonding-based complex formation of (S)-nilvadipine with 4-VPY in chloroform. These results reveal that the (S)-nilvadipine-imprinted EDMA polymers could recognize the template molecule by its molecular shape, and that in addition to this recognition, hydrophobic and hydrogen- bonding interactions seems to play important roles in the retention and chiral recognition of nilvadipine on the 4-VPY- co-EDMA polymers in hydroorganic mobile phases. By optimizing chromatographic conditions such as column temperature and flow rate, the baseline separation of nilvadipine enantiomers was attained with a short analysis time and with a column efficiency comparable to commercially available chiral stationary phases based on a protein, such as ovomucoid or alpha(1)-acid glycoprotein

  Periodic table Coach hat  Life Science peptide mug  Gravity waves shirt

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner