MIPs logo MIPdatabase      Use this space
Custom Search
Reference type: Journal
Authors: Rezeli M, Kilar F, Hjertén S
Article Title: Monolithic beds of artificial gel antibodies.
Publication date: 2006
Journal: Journal of Chromatography A
Volume: 1109
Issue: (1)
Page numbers: 100-102.
DOI: 10.1016/j.chroma.2005.11.076
Alternative URL: http://www.sciencedirect.com/science/article/B6TG8-4HTBM10-5/2/d82e104af21ec616344007595c107e2d

Abstract: The introductions of the continuous beds, now often called monoliths [S. Hjerten, J.-L. Liao, R. Zhang, J. Chromatogr. 473 (1989), 273-275] and the artificial, highly selective gel antibodies against antigens as large as proteins, viruses and cells [J.-L. Liao, Y. Wang, S. Hjerten, Chromatographia 42 (1996), 259-262] were breakthroughs in the design of chromatographic beds. This paper deals with a combination of these two methods, i.e., the artificial gel antibodies have been synthesized in the monolithic mode. As antigen we have used human hemoglobin. A comparison of the ion-exchange chromatograms of the eluates from the monolithic columns shows that the monolith prepared in the presence of hemoglobin adsorbed this protein, but not the other proteins in the sample (ribonuclease A and cytochrome c), i.e., this monolith was selective for hemoglobin, whereas the blank column (prepared in the absence of hemoglobin) had no selective properties, since none of the applied proteins were adsorbed. The diameter of the column was 6 mm, but the same approach to synthesize a monolithic selective bed can very likely also be used for capillaries and microchips
Template and target information: protein, human hemoglobin
Author keywords: continuous bed, monolith, Artificial gel antibodies, hemoglobin, Ion-exchange chromatography

  Mister Benzene Leaving card  Chemistry peptide mug  Beach bunny periodic table shirt

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner