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Reference type: Journal
Authors: Gilar M, Bouvier ESP, Compton BJ
Article Title: Advances in sample preparation in electromigration, chromatographic and mass spectrometric separation methods.
Publication date: 2001
Journal: Journal of Chromatography A
Volume: 909
Issue: (2)
Page numbers: 111-135.
DOI: 10.1016/S0021-9673(00)01108-0

Abstract: The quality of sample preparation is a key factor in determining the success of analysis. While analysis of pharmaceutically important compounds in biological matrixes has driven forward the development of sample clean-up procedures in last 20 years, today's chemists face an additional challenge: sample preparation and analysis of complex biochemical samples for characterization of genotypic or phenotypic information contained in DNA and proteins. This review focuses on various sample pretreatment methods designed to meet the requirements for the analysis of biopolymers and small drugs in complex matrices. We discuss the advances in development of solid-phase extraction (SPE) sorbents, on-line SPE, membrane-based sample preparation, and sample clean-up of biopolymers prior to their analysis by mass spectrometry. (C) 2001 Elsevier Science B.V. All rights reserved

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