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Reference type: Journal
Authors: Hantash J, Bartlett A, Oldfield P, Dénès G, O'Rielly R, Roudiere D, Menduni S
Article Title: Use of an on-line imprinted polymer pre-column, for the liquid chromatographic-UV absorbance determination of carbaryl and its metabolite in complex matrices.
Publication date: 2006
Journal: Journal of Chromatography A
Volume: 1125
Issue: (1)
Page numbers: 104-111.
DOI: 10.1016/j.chroma.2006.05.054
Alternative URL: http://www.sciencedirect.com/science/article/B6TG8-4K5ST56-3/2/77cd847a57d0f9311e9ac5573d545a6d

Abstract: A carbaryl and 1-naphtol molecular imprinted polymers (MIP1 and MIP 2, respectively) were prepared using suspension polymerization and tested for selective and reversible binding to carbaryl and 1-naphtol, respectively. In the suspension polymerization technique used, polymers of methacrylic acid (MAA), highly cross-linked with ethylene dimethacrylate, provide a specific binding sites for the carbaryl molecule and its metabolite when using it as a template to be removed after polymerization. The molecular imprinted polymer with a particle size of ~5 μm were isolated and packed into a pre-column (50 mm x 4.6 mm id) that was used to isolate carbaryl and its metabolite from complex matrices injected on a high performance liquid chromatography system using ultra-violet detection without extensive sample preparation and clean up. The HPLC method had a detection limit of 1.00 ng/mL and a linear response (r2 > 0.98) over the concentration range of 1.00-10.0 ng/mL
Template and target information: carbaryl, 1-naphthol
Author keywords: molecular imprinted polymer, liquid chromatography, pesticides, suspension polymerization, detection, HPLC

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