Abstract: The displacement of non-specific dyes from molecularly imprinted polymers (MIPs) has been used to detect different non-fluorescent analytes. A blank polymer and several imprinted polymers were prepared using methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as a cross-linker. The fiber-optic sensor coated with one polymer was placed in a solution containing dye and analyte to monitor the binding competition of these molecules. The binding properties of the MIP to different analytes were measured using fluorescence. Specifically, the competitive displacement of dye molecule by different analytes has been studied under aqueous environment. It is proposed that the fiber-optic sensor coated with MIPs be used for a wide range of analytes by using the dye displacement strategy.