MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Sun HW, Qiao FX, Liu GY
Article Title: Characteristic of theophylline imprinted monolithic column and its application for determination of xanthine derivatives caffeine and theophylline in green tea.
Publication date: 2006
Journal: Journal of Chromatography A
Volume: 1134
Issue: (1-2)
Page numbers: 194-200.
DOI: 10.1016/j.chroma.2006.09.004
Alternative URL: http://www.sciencedirect.com/science/article/B6TG8-4M4CN42-1/2/ceb82400b2a000a225ab917afface5d8

Abstract: Theophylline imprinted monolithic columns were designed and prepared for rapid separation of a homologous series of xanthine derivatives, caffeine, and theophylline by an in situ thermal-initiated copolymerization technique. Caffeine and theophylline were fully separated both under isocratic and gradient elutions on this kind of monolithic molecularly imprinted polymers (MIP) column. The broad peak showed in isocratic elution could be improved in gradient elution. Some chromatographic conditions such as mobile phase composition, flow rate, and the temperature on the retention times were investigated. Hydrogen bonding interaction and hydrophobic interaction played an important role in the retention and separation. The binding capacity was evaluated by static adsorption and Scatchard analysis, which showed that the dissociation constant (KD) and the maximum binding capacity (Qmax) were 1.50 mol/L, and 236 μmol/g for high affinity binding site, and 7.97 mol/L and 785 μmol/g for lower affinity binding site, respectively. Thermodynamic data (Δ Δ H and Δ Δ S) obtained by Van't Hoff plots revealed an enthalpy-controlled separation. The morphological characteristics of monolithic MIP were investigated by scanning electron microscope, which showed that both mesopores and macropores were formed in the monolith. The present monolithic MIP column was successfully applied for the quantitative determination of caffeine and theophylline in different kinds of green tea
Template and target information: theophylline
Author keywords: molecularly imprinted polymer, monolithic column, Characteristic, caffeine, theophylline, Green tea


  I love MIPs bag  multi MIPs logo mug    Mug featuring the template Theophylline

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner