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Reference type: Journal
Authors: Shi Y, Zhang JH, Shi D, Jiang M, Zhu YX, Mei SR, Zhou YK, Dai K, Lu B
Article Title: Selective solid-phase extraction of cholesterol using molecularly imprinted polymers and its application in different biological samples.
Publication date: 2006
Journal: Journal of Pharmaceutical and Biomedical Analysis
Volume: 42
Issue: (5)
Page numbers: 549-555.
DOI: 10.1016/j.jpba.2006.05.022
Alternative URL: http://www.sciencedirect.com/science/article/B6TGX-4KFV3C6-2/2/f7920df1287d002aca9a52464f0b20c5

Abstract: Non-covalent molecularly imprinted polymers (MIPs) of cholesterol were prepared by UV initiated polymerization. A polymer that had the highest binding selectivity and capability was used as solid-phase extraction (SPE) sorbents for direct extraction of cholesterol from different biological samples (human serum, cow milk, yolk, shrimp, pork and beef). The extraction conditions of molecularly imprinted SPE (MISPE) were optimized and the optimum protocol was: conditioning MISPE cartridges with n-hexane, loading with n-hexane, washing with n-hexane and n-hexane:toluene = 9:1, respectively, then eluting with chloroform:ethanol:acetic acid = 3:1:1. Cholesterol MISPE selectively recognized, effectively trapped and pre-concentrated cholesterol over a concentration range of 10-80 μg/mL. Recoveries ranged from 80.6% to 92.7%, with R.S.D. lower than 9.8%. Under the optimal condition, MISPE recoveries of spiked human serum, yolk, cow milk, shrimp, pork and beef were 91.1%, 80.4%, 86.6%, 78.2%, 81.4% and 80.1%, respectively. Compared with C18 SPE, almost all of the matrix interferences were removed after MISPE, and better baselines and higher selectivity were achieved
Template and target information: cholesterol
Author keywords: Molecularly imprinted polymers, Solid-phase extraction, cholesterol, biological samples


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