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Reference type: Journal
Authors: Rick J, Chou TC
Article Title: Using protein templates to direct the formation of thin-film polymer surfaces.
Publication date: 2006
Journal: Biosensors and Bioelectronics
Volume: 22
Issue: (4)
Page numbers: 544-549.
DOI: 10.1016/j.bios.2006.06.035
Alternative URL: http://www.sciencedirect.com/science/article/B6TFC-4KNMB0N-2/2/a0a5f68c9296b20875155e0922e9d15c

Abstract: Protein imprinted electrodes formed by the cyclic voltammetric deposition of conductive polymers, on screen-printed platinum supports, in the presence of target proteins have been fabricated. An initial layer of polypyrrole was used as a supporting polymer layer, upon which were formed two layers of polyaminophenylboronic acid. The first of these layers was non-imprinted and formed a barrier between the polypyrrole and the outer layer, which was deposited in the presence of a protein template (lysozyme or cytochrome c). After protein extraction, re-binding of the template proteins to their respective imprinted electrodes showed a distinct two-phase binding profile; whereas, binding to control polymers, made in the same way but without the addition of protein templates, showed progressive binding typical of non-specific recognition.Reductions in the observed current transmission due to bonding to the polymer surface of non-conductive protein have been used as a measure of re-binding. It was found that when challenged with 1 part per million protein in solution, the current reductions for the lysozyme and cytochrome c imprinted electrodes were 30.3 and 66.2%, respectively, compared to 4.5 and 29.9% for their respective control electrodes. All measurements carried out at -0.1 V with Ag/AgCl reference
Template and target information: protein, lysozyme, cytochrome c
Author keywords: molecular imprinting, protein, lysozyme, cyclic voltammetry, polymerisation, aminophenylboronic acid, polypyrrole

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