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Reference type: Journal
Authors: Baggiani C, Baravalle P, Giraudi G, Tozzi C
Article Title: Molecularly imprinted solid-phase extraction method for the high-performance liquid chromatographic analysis of fungicide pyrimethanil in wine.
Publication date: 2007
Journal: Journal of Chromatography A
Volume: 1141
Issue: (2)
Page numbers: 158-164.
DOI: 10.1016/j.chroma.2006.12.016
Alternative URL: http://www.sciencedirect.com/science/article/B6TG8-4MKV2NM-4/2/1249e7c90654a968ade83d0ab85df392

Abstract: A method for molecularly imprinted solid-phase extraction (MISPE) of the fungicide pyrimethanil from wine samples has been investigated. The molecular imprinted polymer was obtained by iniferter-mediated grafting on porous chloromethylated polystyrene beads, using methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linker. The imprinted beads were evaluated for use as a solid-phase extraction sorbent, in order to develop the extraction protocol in aqueous standards and red wine samples. The optimised extraction protocol resulted in a reliable MISPE method suitable for HPLC analysis (stationary phase: Cromolith Performance C18 column, 100 mm x 4.6 mm; mobile phase: acetonitrile-water (3:2, v/v), flow-rate: 1.00 ml/min; detection 270 nm). It was selective for pyrimethanil and the related pyrimidinic fungicides cyprodinil and mepanipyrim, while the non-pyrimidinic fungicides benalaxyl, chlozolinate, furalaxyl, iprodione, metalaxyl, nuarimol, procymidone and vinclozolin were not extracted. Recoveries performed on a wine matrix spiked with pyrimethanil at three different concentration levels were reproducible and were in good agreement with the recoveries performed on buffer, coming out between 80 and 90% (85 7.0% at 0.50 μg/ml, 79 1.6% at 2.0 μg/ml and 87 5.6% at 20 μg/ml). Preconcentration and quantitative extraction of pyrimethanil from wine samples was shown to be feasible down to 0.1 μg/ml
Template and target information: pyrimethanil, pyrimidinic fungicides, cyprodinil, mepanipyrim
Author keywords: molecular imprinting, Solid-phase extraction, wine analysis, Fungicide, Pyrimethanil

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