MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Baggiani C, Baravalle P, Giraudi G, Tozzi C
Article Title: Molecularly imprinted solid-phase extraction method for the high-performance liquid chromatographic analysis of fungicide pyrimethanil in wine.
Publication date: 2007
Journal: Journal of Chromatography A
Volume: 1141
Issue: (2)
Page numbers: 158-164.
DOI: 10.1016/j.chroma.2006.12.016
Alternative URL: http://www.sciencedirect.com/science/article/B6TG8-4MKV2NM-4/2/1249e7c90654a968ade83d0ab85df392

Abstract: A method for molecularly imprinted solid-phase extraction (MISPE) of the fungicide pyrimethanil from wine samples has been investigated. The molecular imprinted polymer was obtained by iniferter-mediated grafting on porous chloromethylated polystyrene beads, using methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linker. The imprinted beads were evaluated for use as a solid-phase extraction sorbent, in order to develop the extraction protocol in aqueous standards and red wine samples. The optimised extraction protocol resulted in a reliable MISPE method suitable for HPLC analysis (stationary phase: Cromolith Performance C18 column, 100 mm x 4.6 mm; mobile phase: acetonitrile-water (3:2, v/v), flow-rate: 1.00 ml/min; detection 270 nm). It was selective for pyrimethanil and the related pyrimidinic fungicides cyprodinil and mepanipyrim, while the non-pyrimidinic fungicides benalaxyl, chlozolinate, furalaxyl, iprodione, metalaxyl, nuarimol, procymidone and vinclozolin were not extracted. Recoveries performed on a wine matrix spiked with pyrimethanil at three different concentration levels were reproducible and were in good agreement with the recoveries performed on buffer, coming out between 80 and 90% (85 7.0% at 0.50 μg/ml, 79 1.6% at 2.0 μg/ml and 87 5.6% at 20 μg/ml). Preconcentration and quantitative extraction of pyrimethanil from wine samples was shown to be feasible down to 0.1 μg/ml
Template and target information: pyrimethanil, pyrimidinic fungicides, cyprodinil, mepanipyrim
Author keywords: molecular imprinting, Solid-phase extraction, wine analysis, Fungicide, Pyrimethanil


  Bag featuring the name Claudio spelled out in symbols of the chemical elements  Lab Hero Periodic tablemug  HE-MAN polymer chemistry shirt

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner