MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Namvar A, Warriner K
Article Title: Microbial imprinted polypyrrole/poly(3-methylthiophene) composite films for the detection of Bacillus endospores.
Publication date: 2007
Journal: Biosensors and Bioelectronics
Volume: 22
Issue: (9-10)
Page numbers: 2018-2024.
DOI: 10.1016/j.bios.2006.08.039
Alternative URL: http://www.sciencedirect.com/science/article/B6TFC-4M0BHD1-3/2/0217ad4552e2221254096d84d2432d84

Abstract: The fabrication of Bacillus subtilis endospore imprinted conducting polymer films and subsequent electrochemical detection of bound spores is reported. Imprinted films were prepared by absorbing spores on the surface of glassy carbon electrodes upon which a polypyrrole, followed by a poly(3-methylthiophene), layer were electrochemically deposited. Spore template release was achieved through soaking the modified electrode in DMSO. Binding of endospores to imprinted films could be detected via impedance spectroscopy by monitoring changes in Y'' (susceptance) using Mn(II)Cl2 (0.5 M pH 3) as the supporting electrolyte. Here, the change in Y'' could be correlated to spore densities between 104 and 107 cfu/ml. More sensitive detection of absorbed spores was achieved by following endospore germination via changes in film charge as measured using cyclic voltammetry. Here, imprinted films were submerged in spore suspensions to permit absorption, heat activated at 70 C for 10 min prior to transferring to an electrochemical cell containing germination activators. By using the assay format it was possible to detect 102 cfu/ml. The observed changes in film charge could be attributed to the interaction of the supporting conducting polymer with dipicolinic acid (DPA) and other constituents released from the core in the course of germination. In all cases, it was not possible to regenerate the imprinted films without losing electrode response. In summary, the study has provided proof-of-concept for fabricating microbial imprinted films using conducting polymers
Template and target information: Bacillus subtilis endospore, endospore
Author keywords: imprinted polymer, Bacillus, Endospores, Impedance, conducting polymer

  Eat, sleep, imprint, repeat mug  SMI logo mug  Woman of proper-tea mug

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner