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Reference type: Journal
Authors: Haupt K, Mayes AG, Mosbach K
Article Title: Herbicide assay using an imprinted polymer based system analogous to competitive fluoroimmunoassays.
Publication date: 1998
Journal: Analytical Chemistry
Volume: 70
Issue: (18)
Page numbers: 3936-3939.
DOI: 10.1021/ac980175f

Abstract: A fluorescent ligand displacement assay has been developed for the herbicide 2,4-dichlorophenoxyacetic acid based on a molecularly imprinted polymer. The format of the assay is analogous to competitive fluoroimmunoassay formats and uses a coumarin derivative as a nonrelated fluorescent probe. This avoids the use of radiolabels commonly employed in imprinted polymer-based assays and, thus, the handling of radioactive material. Specificity and sensitivity of the assay are on a par with a radioligand displacement assay using the same molecularly imprinted polymer and radiolabeled 2,4- dichlorophenoxyacetic acid. The assay can be used both in aqueous buffer and in organic solvents, the detection limit being about 100 nM


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