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Reference type: Journal
Authors: Haupt K
Article Title: Molecularly imprinted sorbent assays and the use of non-related probes.
Publication date: 1999
Journal: Reactive and Functional Polymers
Volume: 41
Issue: (1-3)
Page numbers: 125-131.
DOI: 10.1016/S1381-5148(99)00023-1

Abstract: Non-related molecular probes have been employed for the development of competitive equilibrium binding assays for the herbicide 2,4- dichlorophenoxyacetic acid based on a molecularly imprinted polymer. The assays are analogous to competitive solid-phase immunoassays, except that imprinted polymer particles were substituted for the antibodies and non-related molecular probes for the labeled antigen. Three different formats were studied which are based on fluorescence, chemiluminescence or electrochemical detection. Specificity and sensitivity of such systems may be on a par with radioligand displacement assays using the same molecularly imprinted polymer and radiolabeled analyte, providing that the probe binds to the imprinted sites with a certain degree of specificity. (C) 1999 Published by Elsevier Science B.V. All rights reserved

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