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Reference type: Journal
Authors: Rajkumar R, Warsinke A, Mohwald H, Scheller FW, Katterle M
Article Title: Development of fructosyl valine binding polymers by covalent imprinting.
Publication date: 2007
Journal: Biosensors and Bioelectronics
Volume: 22
Issue: (12)
Page numbers: 3318-3325.
DOI: 10.1016/j.bios.2007.03.001
Alternative URL: http://www.sciencedirect.com/science/article/B6TFC-4N6NJW9-1/2/57bc6ad47a17ccff94d1911e07b068e5

Abstract: Molecularly imprinted polymers (MIPs) against fructosyl valine (Fru-Val), the N-terminal constituent of hemoglobin A1c β-chains, were prepared by cross-linking of β-d-Fru-Val-O-bis(4-vinylphenylboronate) with an excess of ethylene glycol dimethacrylate (EDMA) or trimethylolpropane trimethacrylate (TRIM). Control MIPs were prepared in analogy by cross-linking the corresponding vinylphenylboronate esters of fructose and pinacol. After template extraction batch rebinding studies were performed using different pH values and buffer compositions. The Fru-Val imprinted TRIM cross-linked polymer binds about 1.4 times more Fru-Val than the fructose imprinted polymer and 2.7 times more Fru-Val than pinacol imprinted polymer. The highest imprinting effect was obtained in 100 mM sodium carbonate/10% methanol (pH 11.4). The TRIM cross-linked Fru-Val imprinted polymer showed a better specificity than the EDMA cross-linked polymer. The binding of valine was very low. Thermo gravimetric analysis indicated that the generated Fru-Val imprinted polymer has high thermo stability. No change in binding was observed after incubation of the polymers in buffer at 80 C for 36 h. Since the functional group of the polymers (phenyl boronic acid) targets the sugar part of Fru-Val the imprint technique used should also be applicable for the development of MIPs against other glycated amino acids and peptides
Template and target information: fructosyl valine, Fru-Val, β-d-Fru-Val-O-bis(4-vinylphenylboronate)
Author keywords: Fructosyl valine, HbA1c, molecularly imprinted polymer (MIP), glycated hemoglobin, trimethylolpropane trimethacrylate

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