Abstract: Chlortetracycline hydrochloride can enhane the chemiluminescence of Ce(IV) and Rh6G in acidic medium. So novel molecularly imprinted polymer solid-phase extraction (MISPE) method combined with flow-injection chemiluminescence for determining chlortetracycline hydrochloride was developed. The MIP was synthesized by using tetracycline as the imprinting molecule which has a structure similar to that of chlortetracycline. A polytetrafluoroe-thylene(PTFE) tube packed with the particles of MIP was employed as an MISPE micro-column and was connected to the sampling loop of the eight-way injection valve for the preconcentration and extraction of chlortetracycline hydrochloride. The eluent was used as the carrier for extracting the adsorbed chlortetracycline hydrochloride to react with a mixture of cerium(IV) and rhodamine6G in the flow cell to produce strong CL. Intensity of CL is linearly changed with the concentration of chlortetracycline hydrochloride from 2×10-8 g/mL to 1×10-6 g/mL and the limit detection is 4×10-9 g/mL(3σ). This method has been successfully applied to the determination of chlortetracycline hydrochloride in liver of chicken sample.
Template and target information: chlortetracycline hydrochloride
Author keywords: molecularly imprinted polymer, solid phase extraction, chemiluminescence, flow-injection, Chlortetracycline