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Reference type: Journal
Authors: Li ZW, Liu SB, Yang GL, Li BZ
Article Title: Fast Separation of Nicotinamide and Nicotinic Acid with Molecularly Imprinted Monolithic Column.
Publication date: 2005
Journal: Chinese Journal of Chromatography
Volume: 23
Issue: (6)
Page numbers: 622-625.
Alternative URL: http://www.chrom-china.com/EN/article/downloadArticleFile.do?attachType=PDF&id=9893

Abstract: Nicotinamide has been employed as template for the preparation of molecularly imprinted monolithic polymer (MIP) which was used as liquid chromatographic stationary phase. It showed that the nicotinamide-MIP monolithic column was capable of recognizing the difference between nicotinamide and nicotinic acid, while the non-imprinted polymer had no such ability. The content of organic solvent, flow rate and pH of the mobile phase were investigated in the experiments. The following high performance liquid chromatographic conditions were selected: column, 50 mm x 4.6 mm i.d.; mobile phase, water; flow rate, 7.0 mL/min; experiment temperature, room temperature. The resolution between nicotinamide and nicotinic acid was 1.8. The results showed that the two compounds could be separated by the nicotinamide-MIP monolithic column. The column is very useful for the enrichment and detection of nicotinamide in body fluids.
Template and target information: nicotinamide
Author keywords: molecularly imprinted polymer, monolithic column, fast separation, nicotinamide, Nicotinic acid

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