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Reference type: Journal
Authors: Hirayama K, Kameoka K
Article Title: Synthesis of polymer particles with specific binding sites for lysozyme by a molecular imprinting technique and its application to a quartz crystal microbalance sensor.
Publication date: 2000
Journal: Bunseki Kagaku
Volume: 49
Issue: (1)
Page numbers: 29-33.
DOI: 10.2116/bunsekikagaku.49.29
Alternative URL: https://www.jstage.jst.go.jp/article/bunsekikagaku/49/1/49_1_29/_article

Abstract: Acrylic acid and the acrylamide were radically polymerized with (NH4)(2)S2O8 in a phosphate buffer containing lysozyme, surface- modified silica beads, and cross-linkeres. A polymer layer having a recognition site to lysozyme on a silica-gel surface was formed using a molecular imprinting technique. The modified silica gel was able to grasp lysozyme selectively compared with non-modified silica gel. Even after an operation of binding and releasing of lysozyme to the modified silica-gel has been repeated several times, the rebinding ability does not decrease. A sensor that use the quartz crystal microbalance method, which involves a polymer layer having a recognition site to lysozyme, was made. The quartz crystal was coated with a polymer layer having a recognition site to lysozyme and set to the cell. When a lysozyme aqueous solution was dropped onto the cell, good sensitivity and response a ere shown. It turns out that it can be used as a sensor film
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