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Abstract: Recent developments in packing materials for high-performance liquid chromatography (HPLC) and capillary electrochromatography (CEC) have been discussed. Silica gel and its derivatives have been used predominantly as column packing materials for HPLC owing to their high resolution and mechanical strength. The fact that silica gel matrixes are chemically unstable under acidic and basic conditions facilitated the developments of silica matrixes with stable polymer-coating and organic- and inorganic-hybrid silica matrixes, which were used for the preparation of reversed-phase sorbents. Studies on titania and zirconia have progressed especially due to their extreme robustness and unique chemo-affinity for organic phosphates. Titania and zirconia also play important roles in phosphoproteome analysis. Various intelligent materials such as temperature- and photo-sensitve stationary phases, bio-affinity sorbents, molecular imprinting polymers and molecular shape-selective packing materials were reported. The invention of monolith columns is a major innovation in column technology. However, our description about monolith columns was shortened because the related topics were reviewed previously by many authors. ® 2007 The Japan Society for Analytical Chemistry
Template and target information: Review - HPLC and CEC
Author keywords: CEC, HPLC, Intelligent material, packing material, silica gel, titania, zirconia