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Reference type: Journal
Authors: Hawkins DM, Ellis EA, Stevenson D, Holzenburg A, Reddy SM
Article Title: Novel critical point drying (CPD) based preparation and transmission electron microscopy (TEM) imaging of protein specific molecularly imprinted polymers (HydroMIPs).
Publication date: 2007
Journal: Journal of Materials Science
Volume: 42
Issue: (22)
Page numbers: 9465-9468.
DOI: 10.1007/s10853-007-1806-4

Abstract: We report the transmission electron microscopy (TEM) imaging of a hydrogel based molecularly imprinted polymer (HydroMIP) specific to the template molecule bovine haemoglobin (BHb). A novel critical point drying based sample preparation technique was employed to prepare the molecularly imprinted polymer (MIP) samples in a manner that would facilitate the use of TEM to image the imprinted cavities, and provide an appropriate degree of both magnification and resolution to image polymer architecture in the > 10 nm range. For the first time, polymer structure has been detailed that tentatively suggests molecularly imprinted cavities, ranging from 5 to 50 nm in size, that correlate (in terms of size) with the protein molecule employed as the imprinting template. The modified critical point drying sample preparation technique used may potentially play a key role in the imaging of all molecularly imprinted polymers, particularly those prepared in the aqueous phase
Template and target information: protein, bovine haemoglobin, BHb

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