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Reference type: Journal
Authors: Bolisay LD, Culver JN, Kofinas P
Article Title: Optimization of Virus Imprinting Methods To Improve Selectivity and Reduce Nonspecific Binding.
Publication date: 2007
Journal: Biomacromolecules
Volume: 8
Issue: (12)
Page numbers: 3893-3899.
DOI: 10.1021/bm7008526

Abstract: Abstract: Molecular imprinting is a technique that creates synthetic materials containing highly specific receptor sites that have an affinity for a target molecule. When large particles such as viruses are imprinted, special consideration must be taken to ensure the formation of complementary cavities. Factors that influence imprint formation, include uniformity of the precross-linked mixture and release of the virus template after cross-linking. In this study, tobacco mosaic virus (TMV) was used as a model virus. Polymervirus aggregates formed when poly(allylamine hydrochloride) (PAA) was mixed with TMV at low polymer concentrations (<0.0001% w/v), but such aggregates were prevented at high polymer concentrations (>25% w/v). Various wash protocols were compared for their ability to remove the virus template from the cross-linked molecularly imprinted polymer (MIP), with sodium hydroxide (1 M) exhibiting the best performance. On the basis of these results, optimized MIPs targeted for TMV virus were synthesized, exhibiting a high affinity to TMV (imprinting factor of 2.3) and low affinity to tobacco necrosis virus, the nontarget virus
Template and target information: tobacco mosaic virus, TMV

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