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Reference type: Journal
Authors: Teke M, SezgintŘrk MK, Dinškaya E, Telefoncu A
Article Title: Two Biosensors for Phenolic Compounds Based on Mushroom (Agaricus bisporus) Homogenate: Comparison in Terms of Some Important Parameters of the Biosensors.
Publication date: 2008
Journal: Preparative Biochemistry and Biotechnology
Volume: 38
Issue: (1)
Page numbers: 51-60.
DOI: 10.1080/10826060701774346
Alternative URL: http://www.informaworld.com/10.1080/10826060701774346

Abstract: Interest in molecular imprinted polymer techniques has increased because they allows for the improvement of some stability characteristics of enzymes. The high stability of molecularly imprinted enzymes for a substrate can make them ideal alternatives as recognition elements for sensors. A bioimprinted mushroom tissue homogenate biosensor was constructed in a very simple way. For this purpose, sulfite was used. The enzyme, polyphenol oxidase, was first complexed by using a competitive inhibitor, sulfite, in aqueous medium and then the enzyme was immobilized on gelatin by crosslinking with glutaraldehyde on a glass electrode surface. Similarly, polyphenol oxidase uncomplexed with sulfite was also immobilized on a glass electrode in the same conditions. The aim of the study was to compare the two biosensors in terms of their repeatability and thermal, pH, and operational stability; also, the linear ranges of the two biosensors were compared with each other
Template and target information: bioimprinting
Author keywords: Enzyme based biosensors, Polyphenol oxidase, Biosensor stability, Bioimprinted biosensors


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