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Reference type: Journal
Authors: Lettau K, Katterle M, Warsinke A, Scheller FW
Article Title: Sequential conversion by catalytically active MIP and immobilized tyrosinase in a thermistor.
Publication date: 2008
Journal: Biosensors and Bioelectronics
Volume: 23
Issue: (7)
Page numbers: 1216-1219.
DOI: 10.1016/j.bios.2007.10.021
Alternative URL: http://www.sciencedirect.com/science/article/B6TFC-4R29600-4/2/7be6530caf394c558c8011af64335334

Abstract: To amplify the heat-signal generated by MIP catalyzed solvolysis of phenylacetate the reaction has been combined for the first time in a reactor with the subsequent oxidation by immobilized tyrosinase. The polymer cleaves the substrate and the released phenol is afterwards converted to o-benzoquinone by the tyrosinase. The separated and sequentially coupled reactions are characterized by the heat generated in a thermistor. The sequential substrate conversion results in a combined heat generation which results a five times higher signal than compared to the polymer alone
Author keywords: catalysts, enzymes, molecularly imprinted polymers (MIPs), Thermal methods


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