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Abstract: Molecularly imprinted polymers(MIP) based on photografting surface-modified polystyrene beads as matrices were prepared with acrylamide as the functional monomer, bovine hemoglobin as the template molecule and N, N'-methylene bisacrylamide as the crosslinker in a phosphate buffer. The results of IR, scanning electron microscope(SEM) and elemental analysis demonstrate the formation of a grafting polymer layer on the Polystyrene-bead surface. Subsequent removal of the template leaves behind cavities on the surface of polymer matrix with a shape and an arrangement of functional groups in complementary binding sites to the original template. And the adsorption studies show that the imprinted polymers have a good adsorption capacity and specific recognition for bovine hemoglobin as the template molecule. Our results demonstrated that the polymer prepared via photografting surface-modified method exhibited a better selectivity for the template. Attempt to employ the new method in molecular imprinting techniques may indroduce new application to MIPs and facillcate the probable protein separation and purification
Template and target information: protein, bovine hemoglobin
Author keywords: molecular imprinting, molecular recognition, Bovine hemoglobin, photografting, iniferter