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Reference type: Journal
Authors: Wu RA, Hu LH, Wang FJ, Ye ML, Zou HF
Article Title: Recent development of monolithic stationary phases with emphasis on microscale chromatographic separation.
Publication date: 2008
Journal: Journal of Chromatography A
Volume: 1184
Issue: (1-2)
Page numbers: 369-392.
DOI: 10.1016/j.chroma.2007.09.022
Alternative URL: http://www.sciencedirect.com/science/article/B6TG8-4PNM491-1/2/c50fd38f70dc520859f58d4c5ae83e0c

Abstract: The column technologies play a crucial role in the development of new methods and technologies for the separation of biological samples containing hundreds to thousands compounds. This review focuses on the development of monolithic technology in micro-column formats for biological analysis, especially in capillary liquid chromatography, capillary electrochromatography and microfluidic devices in the past 5 years (2002-2007) since our last review in 2002 on monoliths for HPLC and CEC [2]. The fabrication and functionalization of monoliths were summarized and discussed, with the aim of presenting how monolithic technology has been playing as an attractive tool for improving the power of existing chromatographic separation processes. This review consists of two parts: (i) the recent development in fabrication of monolithic stationary phases from direct synthesis to post-functionalization of the polymer- and silica-based monoliths tailoring the physical/chemical properties of porous monoliths; (ii) the application of monolithic stationary phases for one- and multi-dimensional capillary liquid chromatography, fast separation in capillary electro-driven chromatography, and microfluidic devices
Author keywords: review, monoliths, column technology, chromatographic separation, stationary phases

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