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Reference type: Journal
Authors: Fu GQ, Yu H, Zhu J
Article Title: Imprinting effect of protein-imprinted polymers composed of chitosan and polyacrylamide: A re-examination.
Publication date: 2008
Journal: Biomaterials
Volume: 29
Issue: (13)
Page numbers: 2138-2142.
DOI: 10.1016/j.biomaterials.2008.01.019
Alternative URL: http://www.sciencedirect.com/science/article/B6TWB-4RTTKV2-4/2/d8347449d43582d0425e74b304b9c1b0

Abstract: In this paper the imprinting effects recently reported with protein-imprinted polymers based on chitosan and polyacrylamide are re-assessed. Molecularly imprinted polymers (MIPs) from which the embedded template molecules were removed by washing with a solution of sodium dodecyl sulfate and acetic acid were prepared. In batch template rebinding experiments, the MIPs displayed quite high template binding capacity as reported previously. However, the non-imprinted polymers (NIPs), after washing with the same solution, also showed large binding capacity nearly equal to that of the MIPs. X-ray diffraction and scanning electronic microscope investigations confirmed remarkable property changes of the NIPs after the washing process. These findings indicate that the non-specific adsorption resulting from the template removing process rather than the imprinted sites generated on the MIPs themselves may account for the high template rebinding capacity of the reported protein-imprinted polymers
Author keywords: molecular imprinting, protein, protein adsorption, chitosan, hydrogel, polyacrylamide

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