MIPdatabase
Authors: Long Y, Xing XC, Han RF, Sun Y, Wang Y, Zhao Z, Mi HF
Article Title: Two-step purification of low-content cellular protein using protein-imprinted polymers.
Publication date: 2008
Journal: Analytical Biochemistry
Volume: 380
Issue: (2)
Page numbers: 268-275.
DOI: 10.1016/j.ab.2008.05.045
Alternative URL: http://www.sciencedirect.com/science/article/B6W9V-4SNNT7R-2/1/9aa736637cd9a1b72e1e774f9afecf86
Abstract: We introduce a new method, based on molecular imprinting, for purification of low-content cellular protein. This is a combination method that uses two types of protein-imprinting polymers (PIPs) synthesized with limited-length polymer chains that contain randomly distributed recognition sites, namely assistant recognition polymer chains, and uses cloned bacterial protein as a template. The low-content cellular target protein was purified from cell extract by this method. This is believed to be the first time that low-content cellular protein has been purified by using PIPs and with only two steps
Template and target information: protein, pig cyclophilin 18, pCyP18
Author keywords: protein-imprinted polymer (PIP), assistant recognition polymer chains (ARPCs) with positively charged recognition sites, template of cloned bacterial protein, enrichment of low content cellular protein
Join the Society for Molecular Imprinting
New items RSS feed
View latest updatesSign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.
Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
