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Reference type: Journal
Authors: Long Y, Xing XC, Han RF, Sun Y, Wang Y, Zhao Z, Mi HF
Article Title: Two-step purification of low-content cellular protein using protein-imprinted polymers.
Publication date: 2008
Journal: Analytical Biochemistry
Volume: 380
Issue: (2)
Page numbers: 268-275.
DOI: 10.1016/j.ab.2008.05.045
Alternative URL: http://www.sciencedirect.com/science/article/B6W9V-4SNNT7R-2/1/9aa736637cd9a1b72e1e774f9afecf86

Abstract: We introduce a new method, based on molecular imprinting, for purification of low-content cellular protein. This is a combination method that uses two types of protein-imprinting polymers (PIPs) synthesized with limited-length polymer chains that contain randomly distributed recognition sites, namely assistant recognition polymer chains, and uses cloned bacterial protein as a template. The low-content cellular target protein was purified from cell extract by this method. This is believed to be the first time that low-content cellular protein has been purified by using PIPs and with only two steps
Template and target information: protein, pig cyclophilin 18, pCyP18
Author keywords: protein-imprinted polymer (PIP), assistant recognition polymer chains (ARPCs) with positively charged recognition sites, template of cloned bacterial protein, enrichment of low content cellular protein

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