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Reference type: Journal
Authors: Ju JY, Shin CS, Whitcombe MJ, Vulfson EN
Article Title: Imprinted polymers as tools for the recovery of secondary metabolites produced by fermentation.
Publication date: 1999
Journal: Biotechnology and Bioengineering
Volume: 64
Issue: (2)
Page numbers: 232-239.
DOI: 10.1002/(SICI)1097-0290(19990720)64:2<232::AID-BIT12>3.0.CO;2-J

Abstract: Imprinted polymers were synthesized using a mixture of pigments, N- glutamyl-rubropuctamine, and N-glutamyl-monascorubramine (I) as the template, and 2-methacrylamido-6-picoline or 4-aminostyrene as functional monomers, to obtain recognition materials capable of forming hydrogen bonds and charge interactions, respectively, with carboxyl groups of target I in the binding sites. The polymers were prepared thermally at a template loading of 5 mol% using ethylene- glycol dimethacrylate or trimethylolpropane trimethacrylate as crosslinkers and acetonitrile or tetrahydrofuran as porogens. The selective binding of I to both types of polymer was demonstrated, although aminostyrene-based materials showed higher overall adsorption and were studied in more detail. It was shown that the kinetics of binding of I from ethyl-acetate extracts of fermented Monascus sp. was very rapid and virtually all the pigment adsorbed can be released by washing the polymer with ethanol-water mixtures. The feasibility of reusing im printed polymer in consecutive adsorption/desorption cycles was also demonstrated. (C) 1999 John Wiley & Sons, Inc


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