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Reference type: Journal
Authors: Yan H, Tian M, Row KH
Article Title: Determination of enrofloxacin and ciprofloxacin in milk using molecularly imprinted solid-phase extraction.
Publication date: 2008
Journal: Journal of Separation Science
Volume: 31
Issue: (16-17)
Page numbers: 3015-3020.
DOI: 10.1002/jssc.200800315

Abstract: A molecularly imprinted solid-phase extraction procedure was developed for the simultaneous identification of enrofloxacin and its active metabolite, ciprofloxacin, in milk samples. Water-compatible molecularly imprinted polymers synthesized in a water-methanol system show a high degree of cross-reactivity for enrofloxacin and ciprofloxacin in aqueous environments. The imprinted particles were applied as selective sorbents in a solid-phase extraction process focusing upon complex milk matrices, which allowed the matrix compounds present in milk samples to be removed effectively. The extracts were sufficiently clean for further chromatographic analysis, and no interference originating from the biological matrix was observed. The mean recoveries of enrofloxacin and ciprofloxacin from milk sample were 82.6-93.5% and 81.2-94.8%, respectively, with the RSD less than 7.5%. This method is simple and sensitive, and is therefore an alternative tool to the existing HPLC methods for analyzing residual enrofloxacin and ciprofloxacin in biological samples
Template and target information: enrofloxacin, ciprofloxacin
Author keywords: Ciprofloxacin, Enrofloxacin, milk, molecularly imprinted polymer, Selective solid-phase extraction


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