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Reference type: Journal
Authors: Yoshimatsu K, LeJeune K, Spivak DA, Ye L
Article Title: Peptide-imprinted polymer microspheres prepared by precipitation polymerization using a single bi-functional monomer.
Publication date: 2009
Journal: Analyst
Volume: 134
Issue: (4)
Page numbers: 719-724.
DOI: 10.1039/b814967d

Abstract: A single bi-functional monomer, N,O-bismethacryloyl ethanolamine (NOBE), was used in precipitation polymerization system to synthesize molecularly imprinted polymer (MIP) microspheres. Highly specific binding sites were obtained for N-terminal protected neuropeptides, Boc-Leu-enkephalin and Pyr-Leu-enkephalin. The use of NOBE allowed binding sites to be formed in polymer microspheres that are able to recognize target peptides through the consensus C-terminal sequence. The interesting molecular binding results suggest a new approach for peptide analysis combining in situ chemical modification with MIP recognition under non-aqueous conditions.
Template and target information: peptides, Boc-Leu-enkephalin, Pyr-Leu-enkephalin


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