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Reference type: Journal
Authors: Figueiredo EC, de Oliveira DM, de Siqueira MEPB, Arruda MAZ
Article Title: On-line molecularly imprinted solid-phase extraction for the selective spectrophotometric determination of nicotine in the urine of smokers.
Publication date: 2009
Journal: Analytica Chimica Acta
Volume: 635
Issue: (1)
Page numbers: 102-107.
DOI: 10.1016/j.aca.2008.12.045
Alternative URL: http://www.sciencedirect.com/science/article/B6TF4-4VB5K3C-1/2/3e225dbd450c641b506f0330c4a3f385

Abstract: This work describes an on-line molecularly imprinted solid-phase extraction (MISPE) method for spectrophotometric determination of nicotine in urine samples of smokers. This method is based on manganese (VII) to manganese (VI) reduction in an alkaline medium, promoted by nicotine. Two wash solutions (1:4 (v/v) acetonitrile:sodium hydroxide - pH 11.4, and nitric acid - pH 2.5) were employed to circumvent interferences. Aqueous solutions containing nicotine plus different possible concomitants (cotinine, anabasine, norcotinine and caffeine) were tested individually. The analytical calibration curve was prepared in urine samples collected from non-smokers and spiked with nicotine standard from 1.1 to 60 μmol L-1 (r2 > 0.998). The limit of quantification and the analytical frequency were 1.1 μmol L-1 and 11 h-1, respectively. The precision, evaluated using 3, 10 and 30 μmol L-1 nicotine in urine, was 10, 10 and 4% (intra-day precision) and 12, 13 and 5% (inter-day precision), respectively. Accuracy was checked through high performance liquid chromatography and the results did not present significant differences at the 95% confidence level according to the Student's t-test
Template and target information: nicotine
Author keywords: Molecularly imprinted polymers, Spectrophotometry, Solid-phase extraction, nicotine, urine


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