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Reference type: Journal
Authors: Ersöz A, Diltemiz SE, Özcan AA, Denizli A, Say R
Article Title: 8-OHdG sensing with MIP based solid phase extraction and QCM technique.
Publication date: 2009
Journal: Sensors and Actuators B: Chemical
Volume: 137
Issue: (1)
Page numbers: 7-11.
DOI: 10.1016/j.snb.2008.12.050
Alternative URL: http://www.sciencedirect.com/science/article/B6THH-4V8GB3K-1/2/2f213a5bb530a81381374057c6ee36a6

Abstract: 8-Hydroxy-2'-deoxyguanosine (8-OHdG) is one of the most abundant oxidative DNA lesions resulting from reactive oxygen species (ROS). The routine measurement of 8-OHdG in biologic samples is a difficult analytical problem due to the low levels of the analyte and complex matrix. In this study, we have proposed an imprinting method for 8-OHdG recognition and determination by methacryloyl aminoantipyrine-Fe(III) [MAAP-Fe(III)] as a new metal-chelating monomer via metal coordination-chelation interactions. The study includes the solid-phase extraction of blood samples by a new 8-OHdG imprinted sorbent and the measurement of binding interaction of 8-OHdG imprinted quartz crystal microbalance (QCM) sensor via ligand interaction. 8-OHdG imprinted sorbent was prepared by bulk polymerization of MAAP-Fe(III) and N-N'-methylenebisacrylamide. By using a photo-graft surface polymerization technique with irradiation by ultra-violet (UV) light, a thin MIP film was prepared for the detection of 8-OHdG. The determined affinity constant (Ka) were found as 78760áM-1 using 8-OHdG imprinted thin film. The 8-OHdG level in blood serum from a breast cancer patient was determined by the prepared QCM sensor. Lastly, analytical performances of this system was compared with capillary electrophoresis (CE) system
Template and target information: 8-Hydroxy-2'-deoxyguanosine, 8-OHdG
Author keywords: molecular imprinting, MIP, QCM, 8-OHdG

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