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Abstract: Two molecularly imprinted polymers (MIPs), in the physical form of well-defined polymer microspheres, were synthesised via precipitation polymerisation (PP) using an antiepileptic drug, carbamazepine (CBZ), as template molecule, methacrylic acid as functional monomer and either divinylbenzene 80 (DVB-80) or a mixture of DVB-80 and ethylene glycol dimethacrylate (EGDMA) as crosslinking agents. The MIP obtained using DVB-80 alone as crosslinking agent (MIP A) had a narrow particle size distribution (9.5á¦á0.5áμm) and a well-developed permanent pore structure (specific surface area in the dry stateá=á758ám2ág-1), whereas when a mixture of DVB-80 and EGDMA (MIP B) were used as crosslinking agents, the polymer obtained had a broader particle size distribution (6.4á¦á1.8áμm) and a relatively low specific surface area (23ám2ág-1). The molecular recognition character of both polymers was evaluated by means of LC and then a molecularly imprinted solid-phase extraction (MISPE) protocol; CBZ was recognised by both polymers, and useful cross-selectivity for oxcarbazepine (OCBZ), which is the main metabolite of CBZ, also observed. In a detailed bioanalytical study, MIP A was selected in preference to MIP B since MIP A enabled a high volume of sample to be extracted such that lower limits of detection were achievable using this polymer. High recoveries of CBZ and OCBZ were obtained in a MISPE protocol when 50ámL of human urine spiked at 0.2ámgáL-1 were percolated through MIP A (90% and 83%, respectively)
Template and target information: carbamazepine, CBZ, oxcarbazepine, OCBZ
Author keywords: molecularly imprinted polymer, Carbamazepine, Oxcarbazepine, Solid-phase extraction, precipitation polymerisation