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Reference type: Journal
Authors: Zheng SL, Song SQ, Lan H, Qu GR, Li RX, Wu AB, Zhang DB
Article Title: Newly Combined Method of Molecularly Imprinted Solid-Phase Extraction with ELISA for Rapid Detection of Clenbuterol in Animal-Tissue Samples.
Publication date: 2009
Journal: Analytical Letters
Volume: 42
Issue: (3)
Page numbers: 600-614.
DOI: 10.1080/00032710802677134
Alternative URL: http://www.informaworld.com/10.1080/00032710802677134

Abstract: A new method using molecularly imprinted polymers (MIPs) as specific adsorbent materials coupled with ELISA analysis is being reported for the first time for the detection of clenbuterol (CLB) residue in the pig muscles. After optimization of the posttreatments, the total amount of template bleeding in the CLB MIPs was decreased to only 3.0 ng CLB/60 mg MIPs, which is 10 times lower than that of the previous report. Moreover, compared to the methods of C18-ELISA and single ELISA, the combined molecularly imprinted solid-phase extraction (MISPE) - ELISA exhibited high precision and robust accuracy for CLB at all three spiked levels of 0.5, 5.0, and 10.0 ng g-1.
Template and target information: clenbuterol, CLB
Author keywords: clenbuterol, ELISA, Molecularly imprinted polymers

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