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Reference type: Journal
Authors: Prasad BB, Tiwari K, Singh M, Sharma PS, Patel AK, Srivastava S
Article Title: Zwitterionic molecularly imprinted polymer-based solid-phase micro-extraction coupled with molecularly imprinted polymer sensor for ultra-trace sensing of L-histidine.
Publication date: 2009
Journal: Journal of Separation Science
Volume: 32
Issue: (7)
Page numbers: 1096-1105.
DOI: 10.1002/jssc.200800595

Abstract: The proposed L-histidine sensing system composed of a molecularly imprinted solid-phase microextraction component combined with a molecularly imprinted polymer sensor was used to determine critical levels of test analyte in a complex matrix of highly diluted human blood serum without any non-specific sorption and false-positive contributions. The molecularly imprinted polymer was a zwitterionic polymer brush derived from the disodium salt of EDTA and chloranil, grafted to solid-phase microextraction material. The hyphenated approach was able to detect L-histidine quantitatively with a limit of detection as low as 0.0435 ng/mL (RSD = 0.2%, S/N = 3)
Template and target information: L-histidine
Author keywords: L-Histidine, Human blood serum, Molecularly imprinted polymer brush, Molecularly imprinted polymer-sensor, solid-phase microextraction


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