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Reference type: Journal
Authors: Prasad BB, Tiwari K, Singh M, Sharma PS, Patel AK, Srivastava S
Article Title: Ultratrace analysis of uracil and 5-fluorouracil by molecularly imprinted polymer brushes grafted to silylated solid-phase microextraction fiber in combination with complementary molecularly imprinted polymer-based sensor.
Publication date: 2009
Journal: Biomedical Chromatography
Volume: 23
Issue: (5)
Page numbers: 499-509.
DOI: 10.1002/bmc.1145

Abstract: Main inborn errors of metabolism diagnosable through uracil (Ura) analysis and the therapeutic monitoring of toxic 5-fluorouracil (5FU) in dihydro pyrimidine dehydrogenase (DPD) deficient patients require a sensitive, reproducible, selective and accurate method. In this work, an artificial receptor in the format of molecularly imprinted polymer (MIP) brush lsquografted torsquo the surface of sol-gel immobilized on cost-effective homemade solid-phase microextraction (SPME) fibers, individually imprinted with either of Ura and 5FU, was used in combination with a voltammetric sensor duly modified with the same MIP. This combination provided up to 10- and 8.4-fold preconcentrations of Ura and 5FU, respectively, which was more than sufficient for achieving stringent detection limits in the primitive diagnosis of uracil disorders and fluoropyrimidine toxicity in DPD-deficient patients. The proposed method permits the assessment of Ura and 5FU plasma concentrations with detection limits pf 0.0245 and 0.0484 ng mL-1 (RSD = 1.0-2.5%, S/N = 3), respectively, without any problems of non-specific false-positives and cross-reactivities in complicated matrices of biological samples. Copyright © 2008 John Wiley & Sons, Ltd
Template and target information: uracil, Ura, 5-fluorouracil, 5FU
Author keywords: Molecularly imprinted polymer brush, solid-phase microextraction, Molecularly imprinted polymer-sensor, uracil, 5-fluorouracil, human blood plasma

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