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Reference type: Journal
Authors: Turan E, zetin G, Caykara T
Article Title: Dependence of Protein Recognition of Temperature-Sensitive Imprinted Hydrogels on Preparation Temperature.
Publication date: 2009
Journal: Macromolecular Bioscience
Volume: 9
Issue: (5)
Page numbers: 421-428.
DOI: 10.1002/mabi.200800273

Abstract: Temperature-sensitive imprinted and non-imprinted hydrogels composed of N-isopropylacrylamide (NIPA) and 2-acrylamido-2-methyl-propanosulfonic acid (AMPS) have been prepared by free-radical crosslinking copolymerization in aqueous solution at three different temperatures: 10C (below the lower critical solution temperature, LCST), 33C (at the LCST), and 40C (above the LCST). Myoglobin (Mb, MW 17 kDa) is used as the template biomolecule. The effects of the initial concentration and adsorption time over the Mb adsorption capacity of the hydrogels have been analyzed and found to be strongly dependent on the preparation temperature (Tprep). The maximum Mb adsorption for the imprinted hydrogel prepared at 10C is 97.402.35 mg Mbg-1 dry gel in 0.32 mgmL-1 Mb solution at 22C. Moreover, batch adsorption equilibrium and selectivity studies have been performed using a reference molecule, hemoglobin (Hb, MW 65 kDa). The imprinted hydrogels have a 2.8-3.3 times higher adsorption capacity for Mb than the non-imprinted hydrogels prepared at the same Tpreps, and also have a 1.8-2.7 times higher selectivity for the imprinted molecule
Template and target information: protein, myoglobin, Mb
Author keywords: hemoglobin, hydrogels, molecularly imprinting, myoglobin, preparation temperature

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