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Reference type: Journal
Authors: Kim JM, Ahn KD, Wulff G
Article Title: Cholesterol esterase activity of a molecularly imprinted polymer.
Publication date: 2001
Journal: Macromolecular Chemistry And Physics
Volume: 202
Issue: (7)
Page numbers: 1105-1108.
DOI: 10.1002/1521-3935(20010401)202:7<1105::AID-MACP1105>3.0.CO;2-P

Abstract: A molecularly imprinted polymer with cholesterol esterase activity was prepared. Polymerization of N,N-diethyl(4-vinylphenyl)amidine in the presence of the phosphate transition state analogue 2 followed by removal of the print molecule generated a catalytically active polymer with Km of 3.7 mM and kcat of 2.210-4 min-1, respectively. A relative rate enhancement of 27-fold over the background buffer uncatalyzed reaction and of 2.4-fold in the presence of the control polymer were observed.

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