Authors: Kim JM
, Ahn KD
, Wulff G
Cholesterol esterase activity of a molecularly imprinted polymer.
Publication date: 2001
Journal: Macromolecular Chemistry And Physics
Abstract: A molecularly imprinted polymer with cholesterol esterase activity was prepared. Polymerization of N,N-diethyl(4-vinylphenyl)amidine in the presence of the phosphate transition state analogue 2 followed by removal of the print molecule generated a catalytically active polymer with Km of 3.7 mM and kcat of 2.2·10-4 min-1, respectively. A relative rate enhancement of 27-fold over the background buffer uncatalyzed reaction and of 2.4-fold in the presence of the control polymer were observed.
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