MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Demircelik AH, Andaç M, Andaç CA, Say R, Denizli A
Article Title: Molecular Recognition-Based Detoxification of Aluminum in Human Plasma.
Publication date: 2009
Journal: Journal of Biomaterials Science-Polymer Edition
Volume: 20
Issue: (9)
Page numbers: 1235-1258.
DOI: 10.1163/156856209X452971

Abstract: Molecular recognition-based Al3+-imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-L-glutamic acid) (PHEMAGA-Al3+) beads were prepared to be used in selective removal of Al3+ out of human plasma overdosed with Al3+ cations. The PHEMAGA-Al3+ beads were synthesized by suspension polymerization in the presence of a template-monomer complex (MAGA-Al3+). The specific surface area of PHEMAGA-Al3+ beads was found to be 55.6 m(2)/g on the average. The MAGA content in the PHEMAGA-Al3+ beads were found to be 640 mu mol/g polymer. The template Al3+ cations could be reversibly detached from the matrix to form PHEMAGA-Al3+ using a 50 mM solution of EDTA. The Al3+-free PHEMAGA-Al3+ beads were then exposed to a selective separation procedure of Al3+ out of human plasma, which was implemented in a continuous system by packing the beads into a separation column (10 cm long with an inner diameter of 0.9 cm) equipped with a water jacket to control the temperature. The Al3+ adsorption capacity of the PHEMAGA-Al3+ beads decreased drastically from 0.76 mg/g polymer to 0.22 mg/g polymer as the flow rate was increased from 0.3 ml/min to 1.5 ml/min. The relative selectivity coefficients of the PHEMAGA-Al3+ beads for Al3+/Fe3+, Al3+/Cu2+ and Al3+/Zn2+ were found to be 4.49, 8.95 and 32.44 times greater than those of the non-imprinted PHEMAGA beads, respectively. FT-IR analyses on the synthesized PHEMAGA-Al3+ beads reveals monodentate and bidentate binding modes of Al3+ in complex with the carboxylate groups of the glutamate residues. Density functional theory computations at the B3LYP/6-31G(d,p) basis set suggests that structured water molecules play essential role in the stability of the monodentate binding mode in 1:1 PHEMAGA-Al3+ complexes. The PHEMAGA-Al3+ beads were recovered and reused many times, with no significant decrease in their adsorption capacities. (C) Koninklijke Brill NV, Leiden, 2009
Template and target information: Al3+
Author keywords: molecular recognition, ion imprinting, aluminum removal, metal detoxification, affinity binding


  Silicon periodic table shirt  Mug featuring the name Adil spelled out in the single letter amino acid code  I shop perodically customisable tote bag

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner