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Reference type: Journal
Authors: Andersson LI, Abdel-Rehim M, Nicklasson L, Schweitz L, Nilsson S
Article Title: Towards molecular-imprint based SPE of local anaesthetics.
Publication date: 2002
Journal: Chromatographia
Volume: 55
Issue: (Supplement 1)
Page numbers: S65-S69.
DOI: 10.1007/BF02493355

Abstract: The use of imprinted polymers for selective solid-phase extraction of local anaesthetics from human plasma was investigated. Methacrylic acid-ethylene glycol dimethacrylate based MIPs were developed by the alternative template approach using a structural analogue, pentycaine, as the template. The influence of the type and concentration of detergent in the buffer on selective and non-specific binding was investigated. Tween 20, Triton X-100 and Brij 35 were found to eliminate non- specific adsorption and leave selective imprint-onalyte binding essentially unaffected. Efficient pre-concert ration of ropivacaine and bupivacaine from plasma samples, both off-line prior to GC and in-line with LC-MS-MS, was demonstrated


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